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  • Liver Biopsy Interpretation: Special Stains - AASLD
    Aside from standard H E, the most common special stains used to assess liver biopsies include trichrome, iron, PAS-D, reticulin, and copper Trichrome is used to assess stage fibrosis and it stains collagen blue
  • Role of special stains in diagnostic liver pathology - PMC
    The stain imparts a blue color to collagen against a red background of hepatocytes and other structures It stains type 1 collagen that is normally present in the portal tracts and vessel walls, but also highlights the presence and distribution of reactive fibrosis as a result of liver injury
  • Special Stains in Interpretation of Liver Biopsies - ResearchGate
    stain and thus can be distinguished from reticulin fibers (Fig 3) In summary, a reticulin stain is useful for demonstrating liver architecture; hepatocyte necrosis and hepatocyte regeneration
  • Collagen immunostains can distinguish capsular fibrous tissue . . .
    Collagen IV, possibly with III or VI in addition to the routine Trichrome and hematoxylin and eosin stain, is useful in differentiating capsular fibrous tissue from septal fibrosis on challenging and fragmented liver biopsies
  • Which stains Special stains in liver pathology - UCSF CME
    • Mean stage 1 0 with H E, 1 69 with trichrome • Trichrome stage was higher in 53 3% • Fibrosis stage was raised by 2 or more points in 17 8% with trichrome stain • The hepatic fibrosis score is significantly underestimated by H E stain in the posttransplant setting in hepatitis C Fractured: Aleta Pippin Special stains: liver pathology
  • Special stains in diagnostic liver pathology - ScienceDirect
    The normal liver shows a preponderance of type I collagen within the capsule and portal tracts (readily demonstrated by trichrome stain) and type III collagen within the space of Disse, 3, 4 serving as the structural backbone surrounding liver-cell plates (demonstrable on reticulin stain)
  • Immunohistochemistry and Special Stains in Liver Pathology
    Trichrome stains, or similar connective tissue stains such as Sirous Red, are critical for evaluating fibrosis In resource-limited environments, a reasonable alternative approach is to cut additional blanks when the H E is cut so that a trichrome stain can be ordered if needed





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