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  • NanoBiT® PPI Starter Systems - Promega Corporation
    The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A positive control pair and a negative control vector Starter systems also include the Nano-Glo Live Cell Assay System
  • scFv-SmBiT - Addgene
    Plasmid scFv-SmBiT from Dr Jennifer Prescher's lab contains the insert scFv - SmBiT86 and is published in Bioconjug Chem 2022 Oct 19;33 (10):1876-1884 doi: 10 1021 acs bioconjchem 2c00348 Epub 2022 Sep 27 This plasmid is available through Addgene
  • Promega N2014, N2015 Protein:Protein Interaction System . . . - manualzz
    The pBiT1 1-C [TK LgBiT], pBiT2 1-C [TK SmBiT], pBiT1 1-N [TK LgBiT] and pBiT2 1-N [TK SmBiT] vectors contain an expression cassette for ampicillin resistance in bacteria, making them suitable for plasmid amplification
  • NanoBiT® PPI Starter Systems - Promega
    The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A positive control pair and a negative control vector Starter systems also include the Nano-Glo Live Cell Assay System
  • pBad-LgBiT-PhoCl1-SmBiT-MBP Sequences (1) - Addgene
    Search by Sequence performs a nucleotide-nucleotide BLAST search against Addgene’s plasmid sequence database BLAST returns plasmids with similarity to the query sequence Results are sorted by E-value, a statistic from BLAST that describes the significance of a match
  • pBad-LgBiT-PhoCl1-SmBiT-MBP - Addgene
    Use text editor or plasmid mapping software to view sequence SnapGene File: Plasmid sequence and SnapGene enhanced annotations Use with SnapGene software or the free Viewer to visualize additional data and align other sequences
  • Novel Cloning Vectors for Stably Expressing . . . - Promega Corporation
    Although transient expression of fusion partners can be sufficient for many experiments, stable cell lines are often needed for maximal reproducibility In this poster, we describe the use of BiBiT-Ready vectors and the BiBiT approach for stable expression of LgBiT and SmBiT fusion proteins
  • NanoBiT Protein:Protein Interaction System - Promega Corporation
    1 Generate constructs encoding LgBiT and SmBiT fusions to protein A and protein B (up to 8 possible constructs) 2 Transiently transfect the different plasmid combinations into cells (up to 8 possible combinations) 3 Screen for an optimal orientation by comparing tool compound treatment to vehicle treatment 4





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