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  • Addgene: Protocol - pLKO. 1 – TRC Cloning Vector
    pLKO 1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs pLKO 1 can be introduced into cells via direct transfection, or can be converted into lentiviral particles for subsequent infection of a target cell line
  • pLKO. 1 Sequence and Map - SnapGene
    Stuffer-containing lentiviral vector for expression of shRNA This plasmid is the official TRC cloning vector Explore Over 2 7k Plasmids: Viral Expression Packaging Vectors | More Plasmid Sets SwaI is typically used at 25°C, but is 50% active at 37°C Efficient cleavage requires at least two copies of the SgrAI recognition sequence
  • pLKO-1载体和shRNA设计 - 知乎
    pLKO 1载体载体是基于 慢病毒 (HIV)的质粒。 这种载体被认为是 生物安全性2级材料 ，由于其改良特性（去除了大量蕴含着HIV病毒毒力的附属基因、最大限度减少病毒微粒的基因组含量，无复制能力及自灭活特性）而无法在感染宿主细胞之后产生病毒，因而可以
  • pLKO. 1 载体图谱和序列 - 纽普生物
    提供 pLKO 1 载体 质粒图谱，全长序列，抗性，大小和元件信息。
  • pLKO. 1 puro载体_质粒图谱 - 优宝生物
    pLKO 1-puro is a transfer and cloning vector of shRNA, originally derived from HIV-1 It contains all the necessary cis-elements for packaging, reverse transcription, and integration, which are required for subsequent production of the lentiviral particles AgeI and EcoRI (only these two sites available)
  • pLKO-1 Vector and shRNA Design Frequently Asked Questions - MilliporeSigma
    The pLKO 1 vector is a lentiviral (HIV)-based plasmid The vector is regarded as a bio-safety level 2 material and safe to use due to its modified features (deletion of a number of accessory genes implicated in the virulence of HIV, minimal genome of the viral particles, non-replicating and self-inactivation features), making it incapable of
  • pLKO. 1-TRC pLK0. 1-TRC质粒图谱、载体图谱、序列、价格 . . .
    pLKO 1-TRC是pLKO 1 puro载体在AgeI和EcoRI限制性内切酶位点中间插入有片段情况下的质粒，可以用来替代pLKO 1 puro载体。
  • Addgene: pLKO. 1 puro
    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E coli The purity and amount is not suitable for direct transfections Storage DNA can be stored at 4℃ (short term) or -20℃ (long term) Addgene has verified this plasmid using Next Generation Sequencing Results are available here


















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