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  • An Introduction to Decalcification - Leica Biosystems
    Decalcification describes the technique for removing minerals from bone or other calcified tissue so that good-quality paraffin sections can be prepared that will preserve all the essential microscopic elements Decalcification is carried out after the specimen has been thoroughly fixed and prior to routine processing to paraffin
  • Science of Decalcification - Fisher Sci
    Decalcification describes the technique for removing calcium minerals from bone or other calcified tissue so paraffin sections can be sectioned Decalcification is carried out between fixation and processing steps What is Decalcification?
  • Decalcification | Gross Pathology Manual - University of Chicago
    Decalcification must be performed such that the tissue can be sectioned in the gross room with a blade and or in histology with a microtome Scant bony fragments (as in marrow margins) can be frozen fresh (without decal) as long as they are small and can be cut with the cryostat
  • Comparison of Different Decalcification Methods Using Rat . . .
    Here, we report results obtained from the decalcification of rat mandibles using 10% ethylenediaminetetraacetic acid (EDTA) at room temperature (RT), 10% EDTA at 37C, 5% nitric acid, and 10% formic acid at RT Decalcification endpoints were determined by microcomputed tomography
  • Bone decalcification - Wikipedia
    Bone decalcification is the softening of bones due to the removal of calcium ions, and can be performed as a histological technique to study bones and extract DNA [1][2] This process also occurs naturally during bone development and growth, and when uninhibited, can cause diseases such as osteomalacia [3]
  • Decalcification - Histology
    Decalcification is a crucial process in histology that involves the removal of calcium deposits from calcified tissues, such as bone and teeth This process is essential for preparing these tissues for subsequent microscopic examination because calcium can interfere with sectioning and staining procedures Why is Decalcification Necessary?
  • Methods and Protocols for Decalcification of Bone Material
    Specimens should be decalcified in hydrochloric acid formic acid working solution 20 times their volume Change to fresh solution each day until decalcification is complete It may take 24 hours up to days or months depending on size of the specimens
  • Laboratory Techniques: Decalcification
    Decalcification is the process whereby calcium salts are removed from bone and other calcified tissues Three general methods are employed to decalcify tissues These include acid hydrolysis, organic chelation, and electrolysis (see Table 2-2) The important points to remember about decalcifying specimens follow: 1
  • Decalcification: What You Need To Know - Leica Biosystems
    Most of us already know that decalcification is the term used for removing mineral from bones or other calcified tissue, such as cartilage This allows for proper press processing, and sectioning of specimens to be used for histological analysis
  • Decalcifying Tissue for Histological Processing - National . . .
    Decalcification is usually carried out between the fixation and processing steps Bone must obviously be processed in this way, but other tissues may also contain calcified areas A variety of agents or techniques have been developed to decalcify tissues, each with advantages and disadvantages





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